Process for isolation of plasma or serum proteins

摘要

The present invention provides a process for the isolation of one or more proteins from a protein solution. This process comprising the steps of: a) providing a protein solution comprising one or more specific proteins and having a preset pH and a preset ionic strength or conductivity, b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and c) obtaining one or more proteins) from the column; wherein the protein solution has been not supplemented with an alcohol.

主权项

1. A process for the large-scale isolation of proteins from a protein solution wherein the protein solution is obtained from human blood, human serum and/or human plasma, and wherein the protein solution has not been supplemented with an alcohol in which process albumin and IgG are separated from one another in two separate protein fractions, said process comprising the steps of:
a) optionally adjusting the pH of the protein solution to a preset pH; b) optionally adjusting the ionic strength or conductivity of the protein solution to a preset ionic strength or a preset conductivity; c) applying said protein solution to an adsorption column comprising an adsorbent, said adsorbent comprises a particle with at least one high density non-porous core, surrounded by a porous material, the adsorbent comprises a particle density of at least 1.5 g/ml and a mean volume particle diameter of at most 150 μm; d) washing one or more of the proteins to be isolated through the adsorbent, without the one or more proteins having been specifically bound to the adsorbent, to obtain a non-bound material fraction; and e) subjecting the adsorbent to an elution buffer to elute at least one of the proteins specifically bound to the adsorbent;wherein either:albumin and IgG are obtained from the adsorbent by stepwise elution in two separate fractions; oralbumin is obtained as non-bound material from the adsorbent and subsequently IgG is obtained from the adsorbent by stepwise elution.