Compositions of active Wnt protein

摘要

Compositions of purified biologically active Wnt proteins are provided. Wnt proteins are found to be hydrophobic and post-translationally modified by addition of a lipid moiety at a conserved cysteine residue. Methods for isolation of Wnt utilize detergents that maintain the solubility of the modified protein.

主权项

1. An in vitro method of maintaining or growing stem cells comprising:
i) contacting a stem cell with a dosage form comprising: a purified, biologically active, substantially homogeneous Wnt protein composition comprising a lipid moiety; and ii) culturing the stem cell in media to maintain or grow stem cells.