| 发明名称 |
METHODS OF CAPTURING SPERM NUCLEIC ACIDS |
| 摘要 |
A method is provided herein, wherein the method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample, comprises contacting a lysis solution to the sample comprising at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate comprises a protamine-DNA complex. The method further comprises applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The antibody binding is followed by capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex. |
| 申请公布号 |
US2016289735(A1) |
申请公布日期 |
2016.10.06 |
| 申请号 |
US201514672358 |
申请日期 |
2015.03.30 |
| 申请人 |
General Electric Company |
发明人 |
Gerdes Michael John;Nelson John Richard;Spooner Patrick McCoy;Lenigk Ralf |
| 分类号 |
C12Q1/68;C12N15/10 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of capturing a sperm deoxyribo nucleic acid (DNA) in a biological sample, comprising:
contacting a lysis solution to the biological sample comprising at least a sperm cell or a sperm cell lysate comprising a protamine-DNA complex to lyse the sperm cell; applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex; capturing the antibody-protamine-DNA complex; and detecting the sperm DNA from the captured antibody-protamine-DNA complex. |
| 地址 |
Schenectady NY US |
