Method of detecting injured nuclear DNA

摘要

An easy method for detecting injured cell nuclear DNA is provided which does not suffer from "pseudo-positive" staining. The method comprises the steps of subjecting a pathological tissue specimen to acid-hydrolysis to selectively hydrolyze any injured DNA thereby to form single-stranded DNA, then treating the specimen with an anti-single-stranded DNA antibody, and subjecting the specimen to morphological inspection to find the presence of the binding of the antibody to the single-stranded DNA. The binding may be discriminated by a labelling marker on the antibody. Alternatively, a labelled secondary antibody which binds to the anti-ssDNA antibody may be used.