Molecular roadblocks for RpoN binding sites

摘要

The present invention relates to, inter alia, a method for repressing transcription and/or gene expression from RpoN binding sites (or promoters) or cryptic promoters upstream of RpoN binding sites. The method comprises providing an agent that specifically and selectively binds to RpoN promoter sequences to inhibit or repress the expression of genes downstream of that promoter; and contacting the RpoN promoter with the agent. Agents for repressing transcription and/or gene expression from RpoN promoters are also provided. The agent can be a composition that binds specifically to the −24, −12, or −24/−12 site(s) for RpoN promoter interference. Synthetic peptides, vectors, and host cells are also provided.

主权项

1. A method for repressing transcription or gene expression from a binding site of interest, wherein the binding site of interest is an RpoN binding site or a cryptic promoter upstream or downstream of an RpoN binding site, comprising the steps of:
(a) providing an agent wherein the agent:
(i) specifically and selectively binds to a sequence comprised in the binding site of interest,(ii) inhibits or represses expression of genes regulated by the binding site of interest, and(iii) comprises a heterologous protein comprising a sequence selected from the group consisting of SEQ ID NOS. 10000-10093; and (b) contacting the agent to the binding site of interest.