| 摘要 |
Methods and apparatuses are described that provide a breakthrough technology for PCR amplification of nucleic acid sequences. The technology permits PCR to be performed without reliance upon temperature cycling and can be applied at a wide range of ambient temperatures, thus freeing the methods from conventional benchtop thermal cycling devices. The methods allow to exercise precise control when desired during replication and amplification, and enable substantial improvements over conventional PCR in a number of parameters including: improved accuracy and fidelity of replication of normal and problematic sequences (GC rich or tandem repeat sequences), greater sequence length, improved overall reaction yield, lower costs, less overall PCR process time, greater portability, and robustness to various environmental parameters, such as temperature, pH, ionic strengths, and contaminants. |
