Method of Producing a Substance with Antimicrobial, Antiviral, and Immunostimulatory Activities, a Substance and a Composition

摘要

A method of producing a substance with antimicrobial, antiviral, and immunostimulatory activities, particularly towards dendritic cells is proposed, provides for using the chopped potato sprouts as plant raw materials are extracted with water, then the aqueous extract is centrifuged, a salt agent is added, and the obtained saline solution is then concentrated using ultrafiltration through a 300-kD filter, then the solution is frozen for 24 hours, thawed, and filtered, and the pellet thus obtained is removed, and the raw peptidoglycan is precipitated from the solution by using an acidic salt agent and is further re-solubilized with alkali, then the alkaline solution is dialyzed against distilled water on a 12 kD filter, and the peptidoglycan having a molecular weight of 500 kD to 17000 kD is purified from the resulting solution using gel permeation chromatography.

主权项

1. A method for producing a substance having antimicrobial, antiviral, and immunostimulatory activity towards dendritic cells, the method comprising:
using chopped potato sprouts as a plant raw material and extracting them with water to obtain an aqueous extract; separating the aqueous extract from a pellet and adding a salt agent to the aqueous extract to obtain a saline solution; concentrating the saline solution by ultrafiltrating it through a 300-kD filter; keeping the saline solution frozen for 24 hours, thawing, and filtering it, and removing a residue; precipitating crude peptidoglycan from the saline solution using an acidic salt agent and re-solubilizing the crude peptidoglycan with alkali to obtain an alkaline solution; dialyzing the alkaline solution against distilled water on a filter to obtain a filtered solution; and obtaining peptidoglycan from the filtered solution using gel permeation chromatography.