| 主权项 |
1. A microfluidic device comprising:
a rotatable platform and a microfluidic structure including a plurality of chambers formed within the platform and valves positioned between the chambers, wherein the microfluidic structure comprises: a first storage chamber accommodating a mixture of two or more types of detection probes, a plurality of reaction chambers, each of the plurality of reaction chambers accommodating two or more types of markers specifically reacting to different types of target materials in a sample, separately by type, and a plurality of detection chambers, each of the plurality of detection chambers being connected to a respectively corresponding one of the plurality of reaction chambers via a respectively corresponding normally closed valve and accommodating a stop solution, wherein the first storage chamber and the plurality of reaction chambers are positioned such that the first storage chamber is positioned closest to a rotation center of the platform and a last one of the plurality of reaction chambers is positioned farthest from the rotation center among the first storage chamber and the plurality of reaction chambers, and each of the plurality of reaction chambers is distanced differently from the rotation center, wherein the detection chambers are positioned to be farther than the reaction chambers connected thereto from the rotation center of the platform, and positioned to be parallel to one another along the circumferential direction of the platform, wherein the plurality of reaction chambers comprise a first reaction chamber positioned closest to the first storage chamber, the first reaction chamber being connected to the first storage chamber through a normally closed valve, wherein the plurality of reaction chambers are connected to one another via normally open valves, and are provided with the sample and the mixture of two or more types of detection probes from the first storage chamber by centrifugal force, and wherein the microfluidic structure further comprises a plurality of second storage chambers, each of the plurality of second storage chambers being connected to a respectively corresponding one of the plurality of reaction chambers via a respectively corresponding normally closed valve and accommodating a substrate solution. |
