BIOMOLECULE SEQUENCING DEVICES, SYSTEMS AND METHODS

摘要

Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

主权项

1. A method for sequencing a biomolecule having a plurality of monomers, comprising:
(a) directing said biomolecule through a channel including a plurality of sets of nanogap electrodes, wherein each set of said plurality of sets of nanogap electrodes includes two nanogap electrodes, and wherein at least a subset of said plurality of sets of nanogap electrodes has different inter-electrode distances; (b) measuring signals with said plurality of sets of nanogap electrodes that correspond to nanocurrents as said biomolecule is directed through said channel, which signals correspond to said plurality of monomers of said biomolecule; and (c) identifying with a computer processor said plurality of monomers by comparing said signals measured in (b) to one or more references.