| 发明名称 |
METHOD FOR THE RECOMBINANT PRODUCTION OF A POLYPEPTIDE IN PROKARYOTIC CELLS |
| 摘要 |
Herein is reported a method for the recombinant production of a polypeptide in E. coli comprising the steps of i) cultivating an NADH dehydrogenase II-deficient E. coli expressing the polypeptide, and ii) recovering the polypeptide from the cell or the cultivation medium. |
| 申请公布号 |
US2016319319(A1) |
申请公布日期 |
2016.11.03 |
| 申请号 |
US201615009588 |
申请日期 |
2016.01.28 |
| 申请人 |
Hoffmann-La Roche Inc. |
发明人 |
SCHANTZ CHRISTIAN |
| 分类号 |
C12P21/00;C12N15/70;C12N9/02 |
主分类号 |
C12P21/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for the recombinant production of a polypeptide in E. coli comprising the following steps:
cultivating an NADH dehydrogenase II-deficient E. coli expressing the polypeptide, and recovering the polypeptide from the cell or the cultivation medium, wherein the polypeptide is not a respiratory chain pathway enzyme or a polypeptide encoded by an antibiotic resistance inducing gene. |
| 地址 |
Little Falls NJ US |
