Accelerated wright-giemsa and may-Grünwald staining methods

摘要

The present disclosure provides methods for carrying out Romanowsky-type stains, specifically Wright-Giemsa and May-Grünwald stains, quickly and efficiently. The methods greatly reduce the overall amount of time required to complete a Wright-Giemsa stain or a May-Grünwald stain of sufficient quality on a biological sample. The subject methods can be applied to both manual and automated staining procedures.

主权项

1. A computer-implemented method for executing instructions stored on a non-transitory computer-readable medium for performing a Wright-Giemsa stain on one or more biological samples in an automated staining system, comprising the following steps performed by one or more computer processors:
applying the biological sample to a substrate in a sample application subsystem; contacting the sample with a fixation reagent bath by transferring the sample from the sample application subsystem to the fixation reagent bath via a sample transfer subsystem; contacting the sample with a Wright-Giemsa staining reagent bath by transferring the sample from the fixation reagent bath to the staining reagent bath via the sample transfer subsystem; and contacting the sample with one or more rinse reagent baths by transferring the sample from the staining reagent bath to the one or more rinse reagent baths via the sample transfer subsystem.