| 摘要 |
The invention may be broadly conceptualized as an approach in which a kind o f window period vaccines (small interference RNAs vaccines), which specifically inhibits viruses influenza virus and foot-and-mouth disease virus (FMDV) that cause the illne ss by infecting respiratory tract, residing and replicating there . The invention comprises: .cndot. Design and generation of a serial of small interference RNA molecule s including siRNAs, shRNAs and microRNAs which are specifically for the genomic highly conserved regions of influenza virus and FMDV. .cndot. Screening of the best small interference RNAs. .cndot. Delivery of viral diseases specific small interference RNAs with cationic lipid complexes as spray vaccines during the window period of viral infection. By imitating viruses' infectious route of entry into an organism, small interference RNAs vaccines could be directly sprayed to the infected respiratory tract during the window period of virus infection. The direct delivery of small interference RNAs in to specific cells would save the usage of small interference RNAs and increase the efficiency of silencing of gene expression in the target organs or tissues and also reduce potential toxicity and other side effects. Small interference RNAs vaccines could inhibit the multiplication of influenza virus and FMDV, degrade their specific mRNAs and prevent their epidemics. Small interference RNAs vaccines as window period vaccines would be the most promising and practical vaccines.
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