发明名称 |
Methylated nucleotide regulation of gene expression |
摘要 |
The present invention provides a method of regulating the expression of the urokinase type plasminogen activator ("uPA") gene in a mammalian cell comprising the steps of: identifying a target nucleotide sequence in a gene of interest, obtaining a methylated antisense oligonucleotide sequence complementary to the target sequence, said oligonucleotide sequence having 5 methylated cytosine in place of unmethylated cytosine in the region complementary to the CpG region in the target nucleotide sequence, and exposing the cell to copies of the oligonucleotide sequence under suitable conditions, such that the oligonucleotide sequence enters the nucleus and interacts with the target sequence to promote methylation of the target nucleotide sequence.
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申请公布号 |
US7316900(B2) |
申请公布日期 |
2008.01.08 |
申请号 |
US20030332542 |
申请日期 |
2003.07.31 |
申请人 |
QUEBEPHARMA RECHERCHE, INC. |
发明人 |
RABBANI SHAFAAT A.;SLEMON CLARKE |
分类号 |
C12Q1/68;G01N33/53;A61K31/7115;A61K38/00;A61P9/00;A61P35/00;A61P35/04;A61P43/00;C07H21/00;C07H21/02;C07H21/04;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/09;C12N15/113;C12P19/34;C12Q1/02;G01N33/566 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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