RECOVERY AND PURITY OF MAGNETICALLY TARGETED CELLS

摘要

Methods and apparatus for separation of rare cells of interest from a biological sample are provided. The present methods and apparatus effectively reduce fragile rare cell exposure to manipulations that adversely impact cell integrity and collection.

主权项

1. A method for isolating cells of interest, comprising;
a) obtaining a biological sample containing said cells of interest using a large gauge needle, thereby minimizing shear forces on said cells, b) concentrating said cells in a solution in the presence of cell integrity preserving inhibitors, c) contacting said cells with an antibody immuno-specific for an antigen on said cells of interest, said antibody being conjugated to a ferrofluid; d) subjecting said cells to conditions suitable for an immunocomplex to form between said cells and said antibody conjugated to said ferrofluid; e) separating immunocomplexed cells by applying a magnetic gradient to said solution, thereby isolating said cells of interest.