MONITORING CELL-TO-CELL INTERACTIONS

摘要

In a method for monitoring cell-to-cell interactions, a quartz crystal microbalance surface is exposed to a medium including a first cell. The first cell is exposed to a sample including a suspect cell. The first cell is activated prior to or simultaneously with the first cellexposure. Frequency and motional resistance changes versus time are measured after each of: surface exposure to the medium, first cell activation prior to the exposure to the sample, and first cell exposure to the sample; or after each of: surface exposure to the medium and simultaneous first cell activation and sample exposure. From the frequency and motional resistance changes versus time, any of i) a level of adhesion of the suspect cell to the activated first cell, ii) a type of the suspect cell, iii) a behavior or activity of the suspect cell is determined, or iv) activation of the first cell is determined.

主权项

1. A method for monitoring cell-to-cell interactions, the method comprising:
exposing a surface of a quartz crystal microbalance to a medium including a first cell; exposing the first cell to a sample including a suspect cell; activating the first cell on the surface either prior to the exposing of the first cell to the sample or simultaneously with the exposing of the first cell to the sample; measuring a frequency change versus time and a motional resistance change versus time:
after each of the exposing of the surface to the medium including the first cell, the activating that takes place prior to the exposing of the first cell to the sample, and the exposing of the first cell to the sample; orafter each of the exposing of the surface to the medium including the first cell and the simultaneous activating and exposing; and from the frequency changes versus time and the motional resistance changes versus time, determining any of i) a level of adhesion of the suspect cell to the activated first cell, or ii) a type of the suspect cell, or iii) a behavior or activity of the suspect cell, or iv) activation of the first cell.