| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide a method for efficiently creating a cloned nonhuman animal including a cloned pig by a somatic cell nucleus direct injection method for injecting a somatic cell nucleus of a nonhuman animal such as a enucleated pig, etc., to the ovum of the nonhuman animal. SOLUTION: A nucleus is removed from a collected swine internal mature ovum and a swine fetal fibroblast nucleus is directly injected to the enucleated recipient ovum. The ovum into which the fetal fibroblast nucleus is injected is subjected to an electric pulse activation treatment, the nucleus transplanted embryo after the activation treatment is subjected to a triple embedding treatment using an embedding agent such as alginic acid gel, etc. The nucleus transplanted embryo subjected to triple embedding treatment is transplanted into a female swine fallopian tube or uterus after miscarriage treatment to prepare a cloned pig. When the nucleus transplanted embryo is transplanted into a female swine fallopian tube or uterus, a plurality of fertilized eggs are mixed with the nucleus transplanted embryo and can be transplanted into a female swine fallopian tube or uterus.</p> |
