发明名称 |
Immobilization of biomolecules on perfluorocarbon surfaces |
摘要 |
Biomolecules such as a ligand or binder for the ligand are securely but reversibly attached to a perfluorocarbon carrier with a water soluble polymer, a perfluorocarbon anchoring group and optionally a linker group. The order of steps for carrying out the attachment can vary. For example, the biomolecule is covalently attached to the polymer followed by covalently attaching the anchoring group and attaching the resultant product to the carrier. Alternatively, the anchoring group is covalently attached to the polymer followed by attaching the resultant product to the carrier and then covalently attaching a biomolecule to the polymer. The polymer may be starch, dextran, agarose, polyethylene glycol or polyvinyl alcohol. An attached ligand or binder for the ligand is useful in affinity separations and immunoassays.
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申请公布号 |
US5270193(A) |
申请公布日期 |
1993.12.14 |
申请号 |
US19910785887 |
申请日期 |
1991.10.24 |
申请人 |
E. I. DUPONT DE NEMOURS AND COMPANY |
发明人 |
EVELEIGH, JOHN W. D.;LOWE CHRISTOPHER ROBIN |
分类号 |
C07K1/22;C12N9/04;G01N33/543;G01N33/545;(IPC1-7):C12N11/06;C12N11/08;G01N33/549 |
主分类号 |
C07K1/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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