| 摘要 |
PURPOSE: To obtain an immobilized enzyme by supporting an ultrathermostableβ-galactosidase derived from Pyrococcus furiosus on a carrier, excellent in durability, hardly eluting the enzyme, capable of sterilizing various germs at a high temperature, useful for processing a food and producing a saccharide compound. CONSTITUTION: Ultrathermostableβ-galactosidase collectable from Pyrococcus furiosus DSM3,638, etc., is supported on an immobilizing carrier (insoluble carrier) stable to heat and physical shocks to give a high-temperature bioreactor. The immobilization can be carried out by a method for subjecting a carboxyl group, etc., of the enzyme to covalent bond formation with a functional group (amino group, etc.) introduced to the immobilizing carrier. |
