Generation of cardiomyocytes from human pluripotent stem cells

摘要

Methods for generating high-yield, high-purity cardiomyocyte progenitors or cardiomyocytes from pluripotent cells are described. Wnt/β-catenin signaling is first activated in pluripotent cells, e.g., by inhibition of Gsk-3 to obtain a first population of cells. Wnt/β-catenin signaling is then inhibited in the first cell population to induce cardiogenesis under fully defined, growth factor free culture conditions.

主权项

1. A method for generating a cell population of human cardiomyocyte progenitors, comprising inhibiting Wnt/β-catenin signaling in a first cell population to obtain a second cell population comprising human cardiomyocyte progenitors, wherein inhibiting comprises culturing the first cell population in the presence of an inhibitor of Wnt/β-catenin signaling, wherein the culturing is substantially free of exogenous growth factors, and wherein the first cell population is obtained by activating Wnt/β-catenin signaling in human pluripotent stem cells by culturing the human pluripotent stem cells in the presence of a Gsk3 inhibitor.