HLA-restricted, peptide-specific antigen binding proteins

摘要

Antigen binding proteins with TCR-like paratopes, that is, with an antigen binding region specific for an HLA-A2 restricted peptide are disclosed. The antigen binding proteins encompass antibodies in a variety of forms, including full-length antibodies, substantially intact antibodies, Fab fragments, F(ab′)2 fragments, and single chain Fv fragments. Fusion proteins, such as scFv fusions with immunoglobulin or T-cell receptor domains, incorporating the specificity of the antigen binding region for each peptide are also contemplated by the invention. Furthermore, immunoconjugates may include antibodies to which is linked a radioisotope, fluorescent or other detectable marker, cytotoxin, or other molecule are also encompassed by the invention. Among other things, immunoconjugates can be used for delivery of an agent to elicit a therapeutic effect or to facilitate an immune effector function.

主权项

1. An antigen-binding protein comprising one of:
(A) an antigen binding region having the amino acid sequence of SEQ ID NO: 5; (B) an antigen binding region comprising a VH and a VL respectively, with the amino acid sequences SEQ ID NOs: 24 and 25; or (C) (i)
(a) a light chain (LC) complementarity determining region 1 (CDR1) comprising the amino acid sequence of SEQ ID NO: 56;(b) a LC CDR2 comprising the amino acid sequence of SEQ ID NO: 58; and(c) a LC CDR3 comprising the amino acid sequence of SEQ ID NO: 65; and
(ii) (a) a heavy chain (HC) complementarity determining region 1 (CDR1) comprising the amino acid sequence of SEQ ID NO: 39;
(b) a HC CDR2 comprising the amino acid sequence of SEQ ID NO: 41; and(c) a HC CDR3 comprising the amino acid sequence of SEQ ID NO: 49.