Nucleic acid construct comprising nucleic acid derived from genome of hepatitis C virus of genotype 3a

摘要

A nucleic acid includes, in the following order, a 5′ untranslated region comprising a particular nucleotide sequence of the genome of hepatitis C virus genotype 3a; a nucleotide sequence encoding a particular amino acid sequence of an NS3 protein, a nucleotide sequence encoding a particular amino acid sequence of an NS4A protein, a nucleotide sequence encoding a particular amino acid sequence of an NS4B protein, a nucleotide sequence encoding a particular amino acid sequence of an NS5A protein, a nucleotide sequence encoding a particular amino acid sequence of an NS5B protein of the hepatitis C virus genotype 3a; and a 3′ untranslated region comprising a particular nucleotide sequence of a genome of hepatitis C virus genotype 3a.

主权项

1. A nucleic acid comprising, in the following order, a 5′ untranslated region comprising a nucleotide sequence of nucleotides 1 to 340 of SEQ ID NO: 1; a nucleotide sequence encoding an amino acid sequence of an NS3 protein of amino acids 1033 to 1663, a nucleotide sequence encoding an amino acid sequence of an NS4A protein of amino acids 1664 to 1717, a nucleotide sequence encoding an amino acid sequence of an NS4B protein of amino acids 1718 to 1978, a nucleotide sequence encoding an amino acid sequence of an NS5A protein of amino acids 1979 to 2430, a nucleotide sequence encoding an amino acid sequence of an NS5B protein of amino acids 2431 to 3021 of SEP ID NO: 14; and a 3′ untranslated region comprising a nucleotide sequence of nucleotides 9407 to 9655 of SEQ ID NO: 1, which are of a genome of a hepatitis C virus of genotype 3a, provided that if the nucleic acid is RNA, thymine (t) in the nucleotide sequence is replaced with uracil (u), and comprising nucleotide mutation(s), wherein the nucleotide mutation(s) include a nucleotide mutation that causes at least one amino acid substitution of the following (a) to (g), as defined on the basis of the amino acid sequence shown in SEQ ID NO: 14:
(a) a substitution of threonine at position 1286 with isoleucine; (b) a substitution of threonine at position 2188 with alanine; (c) a substitution of arginine at position 2198 with histidine; (d) a substitution of serine at position 2210 with isoleucine; (e) a substitution of threonine at position 2496 with isoleucine; (f) a substitution of arginine at position 2895 with glycine; or (g) a substitution of arginine at position 2895 with lysine.