| 摘要 |
PROBLEM TO BE SOLVED: To detect a point mutation by allowing a probe having a sequence complementary to a specific sequence in a nucleic acid and labeled at the both terminal with different fluorochromes causing transfer of a fluorescence resonance energy to coexist with a nucleic acid and measuring the ratio of the luminescent degree of the different fluorochromes. SOLUTION: This method for detecting a point mutation existing in a specific sequence in the target nucleic acid comprises a step for preparing a probe having a base sequence complementary to the two specific sequence in the target nucleic acid, further having one terminal labeled by one of two kinds of fluorochromes causing transfer of a fluorescence resonance energy and the other terminal labeled by the other fluorochrome of the two kinds of the fluorochromes and a step for measuring the ratio of the luminescent degree at the maximum fluorescence-absorbing wave length of one fluorochrome to the luminescent degree at the maximum fluorescence-absorbing wave length of the other fluorochrome while coexisting the probe with the target nucleic acid at a temperature higher than the room temperature to detect the point mutant being in the specific sequence of the target sequence. |
