COMPOSITIONS AND METHODS FOR INCREASING PROTEIN HALF-LIFE IN A SERUM

摘要

Novel antibodies, such as single domain antibodies (sdAbs), or fragments thereof that specifically bind a transferrin are described. Compositions, methods and systems for increasing the half-life of a target protein in a serum using an antibody or fragment thereof against a transferrin are described.

主权项

1. An isolated antibody or fragment thereof that specifically binds a transferrin, the antibody or fragment thereof comprising one or more selected from the group consisting of:
(a) a complementarity determining region (CDR) 1 comprising an amino acid sequence selected from the group consisting of GSGFGINGVI (SEQ ID NO: 1); GSGFGVNGVI (SEQ ID NO: 2); GNVFTIAAMG (SEQ ID NO: 3); GNVFTIAAMA (SEQ ID NO: 4); GNVFTIDAMG (SEQ ID NO: 5); GSVFSIDAMG (SEQ ID NO: 6); GNVFGIDAVG (SEQ ID NO: 7); GSIFSIKVMG (SEQ ID NO: 8); and GSIFPLNDMG (SEQ ID NO: 9); (b) a CDR2 comprising an amino acid sequence selected from the group consisting of LIKSDGYTNYRESVKG (SEQ ID NO: 10); LIKSDGYTNYRESVRG (SEQ ID NO: 11); GITTGGSTNYADSVKG (SEQ ID NO: 12); GMTNGGKTNYADSVKG (SEQ ID NO: 13); AMTNAGSTNYADSVKG (SEQ ID NO: 14); ATTTSGSSTNYADSVKG (SEQ ID NO: 15); DITSGGSTDYSDSVKG (SEQ ID NO: 16); and TITRGGTTNYADSVKG (SEQ ID NO: 17); and (c) a CDR3 comprising an amino acid sequence selected from the group consisting of PGVP (SEQ ID NO: 18); VTKWAARVGGSAEYE (SEQ ID NO: 19); RSKLIATINNPYDY (SEQ ID NO: 20); RSKLIARINNPYEY (SEQ ID NO: 21); RPKQATLIRDDY (SEQ ID NO: 22); DLGCSGAGSCPDY (SEQ ID NO: 23); and DNRVGGSY (SEQ ID NO: 24).