Norovirus immunogens and related materials and methods

摘要

The inventors have successfully developed a recombinant vesicular stomatitis virus which expresses the major capsid protein of human norovirus. Infection of mammalian cells with the recombinant vesicular stomatitis virus resulted in production of high level of human norovirus virus-like particles. Importantly, the inventors further demonstrated that recombinant vesicular stomatitis virus expressing the major capsid protein of human norovirus displayed attenuated virulence in mice and elicited a high level of human norovirus-specific humoral, cellular, and mucosal immune responses in mice model. Therefore, norovirus-immunogenic compositions are herein provided, as are materials and methods useful to make and use the compositions.

主权项

1. A live recombinant vesicular stomatitis virus (VSV)-vector vaccine composition for a human norovirus, the composition comprising a virus encoding a foodborne viral immunogen inserted between glycoprotein and polymerase genes of a VSV genome, wherein the foodborne viral immunogen is a major capsid protein of a norovirus, or an immunogenic fragment thereof;
wherein the insertion attenuates or eliminates VSV virulence, wherein the encoded foodborne virus immunogen forms a virus-like particle (VLP) when the norovirus immunogen is expressed, and wherein the VLP is capable of being formed in vivo subsequent to norovirus immunogen expression from the VSV-vector vaccine composition.