| 发明名称 | Glucose dehydrogenase | ||
| 摘要 | A modified pyrroloquinoline quinone glucose dehydrogenase that exhibits a high selectivity for glucose is provided. A modified pyrroloquinoline quinone glucose dehydrogenase is disclosed in which the amino acid residue G at Position 99 of a pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 1, or the amino acid residue G at Position 100 of the pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 3, is substituted by the amino acid sequence TGZN (where Z is SX, S, or N and X is any amino acid residue). The modified PQQGDH of the present invention may additionally comprise one or more mutations selected from the group consisting of Q192G, Q192A, or Q192S; L193X; E277X; A318X; Y367A, Y367F, or Y367W; G451C; and N452X (where X is any amino acid residue). | ||
| 申请公布号 | US9410184(B2) | 申请公布日期 | 2016.08.09 |
| 申请号 | US201414520807 | 申请日期 | 2014.10.22 |
| 申请人 | ARKRAY, Inc.;Ultizyme International Ltd. | 发明人 | Sode Koji |
| 分类号 | C12N9/02;C12Q1/54;C12Q1/26;C07H21/04;C12N9/04;C12Q1/00 | 主分类号 | C12N9/02 |
| 代理机构 | Morgan, Lewis & Bockius LLP | 代理人 | Morgan, Lewis & Bockius LLP |
| 主权项 | 1. A method of analyzing glucose level using a modified pyrroloquinoline quinone glucose dehydrogenase (PGGGDH), wherein the amino acid residue G at Position 100 of the PQQGDH represented by SEQ ID NO: 3 is substituted by the amino acid sequence TGZN where Z is SX, S, or N, and X is any amino acid residue, and optionally from 1 to 10 of amino acid residues at Positions 1 to 99 or at Positions 101 to 480 of SEQ ID NO: 3, are substituted with any other amino acid residue(s). | ||
| 地址 | Kyoto JP | ||