Methods and compositions for expressing functional class XIV myosin

摘要

The invention, in part, includes methods and compounds useful to prepare and functional class XIV myosin. Functional class XIV myosin prepared using methods of the invention may be useful to screen for and identify compounds that inhibit and treat parasitic infections and contamination.

主权项

1. A method of producing a functional class XIV myosin polypeptide, the method comprising,
co-expressing three or more polynucleotides in a heterologous expression-system cell, wherein the three or more polynucleotides comprise a class XIV heavy chain polypeptide-encoding polynucleotide, a first myosin light chain polypeptide-encoding polynucleotide, and a parasite co-chaperone polypeptide-encoding polynucleotide, wherein the three or more polynucleotides are co-expressed in the cell under conditions suitable to produce a functional class XIV myosin polypeptide comprising the class XIV heavy chain polypeptide and the first myosin light chain polypeptide.