发明名称 |
Recombinant non-pathogenic marek's disease virus constructs encoding infectious laryngotracheitis virus and newcastle disease virus antigens |
摘要 |
Recombinant multivalent non-pathogenic Marek's Disease virus constructs that encode and express both Infectious Laryngotracheitis Virus and Newcastle Disease virus protein antigens, and methods of their use in poultry vaccines. |
申请公布号 |
US9409954(B2) |
申请公布日期 |
2016.08.09 |
申请号 |
US201414533666 |
申请日期 |
2014.11.05 |
申请人 |
Intervet Inc. |
发明人 |
Cook Stephanie;Morsey Mohamad;Petersen Gary;Sondermeijer Paulus Jacobus Antonius |
分类号 |
C07K14/005;C12N7/00;A61K39/12;C12N15/86;A61K39/245;A61K39/00 |
主分类号 |
C07K14/005 |
代理机构 |
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代理人 |
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主权项 |
1. A recombinant nonpathogenic Marek's Disease Virus (rMDVnp) comprising a first nucleic acid inserted in a first nonessential site in the rMDVnp genome and a second nucleic acid inserted in a second nonessential site in the rMDVnp genome;
wherein the first nucleic acid comprises both a nucleotide sequence that encodes an Infectious Laryngotracheitis Virus glycoprotein D (ILTVgD) and a nucleotide sequence that encodes an Infectious Laryngotracheitis Virus glycoprotein I (ILTVgl); wherein the second nucleic acid comprises a nucleotide sequence that encodes a Newcastle Disease Virus fusion protein (NDV F); wherein the first nonessential site and the second nonessential site are either both the UL54.5 site or only the first nonessential site is the US2 site; and wherein the rMDVnp is not a recombinant avian herpesvirus comprising a Marek's disease virus (MDV) unique short viral genome region, a herpesvirus of turkeys (HVT) unique long viral genome region, and the repeat viral genome regions of the HVT. |
地址 |
Madison NJ US |