| 摘要 |
<p>RNA polymerase transcription promoters containing compounds represented by general formula (I) or salts thereof. A method for determining the base sequence of a DNA which comprises obtaining a nucleic acid transcriptional product by using RNA polymerase with the use of a DNA fragment as a template, separating the thus obtained nucleic acid transcriptional product and reading the nucleic acid sequence from the fraction thus separated, wherein the above-mentioned nucleic acid transcription reaction is carried out in the presence of at least one compound selected from the group consisting of the compounds represented by general formula (I). Since these polyamine compounds have an excellent activity of promoting the transcriptional activity of RNA polymerase, use thereof in the method of determining a DNA base sequence with the use of RNA polymerase makes it possible to prolong the DNA sequence length which can be read in a single sequencing.</p> |
