NOVEL POOLING AND DECONVOLUTION STRATEGY FOR LARGE SCALE SCREENING

摘要

Embodiments of the present invention provide methods, systems, kits, and apparatus for data analysis to detect interactions between atoms, molecules and/or cells. One embodiment of the present invention provides for a novel pooling-deconvolution strategy that can dramatically decrease the effort required to generate large-scale data sets. This "PI-Deconvolution" strategy employs an N-digit base-X imaginary coding of XN probe proteins (baits or preys) and allows the screening of the baits in X*N pools, with N replicates for each bait. Deconvolution of proteins with their binding partners can be achieved by reading the prey's profile from the X*N experiments. The method can be used to screen XN bait or prey proteins.