METHODS FOR GENERATING AUGMENTED STEM CELL-DERIVED BETA CELLS AND USES THEREOF

摘要

Disclosed herein are methods for generating augmented SC-β cells, and isolated populations of augmented SC-β cells for use in various applications, such as cell therapy.

主权项

1. A method for generating augmented stem cell-derived β (SC-β) cells, the method comprising contacting a cell population comprising SC-β cells, or precursors thereof, with an effective amount of an agent that decreases the level and/or activity of AXL receptor tyrosine kinase (AXL) and an antioxidant for a period of time sufficient for the level of MAFA gene expression to increase in the SC-β cells to at least 2 fold greater than the level of MAFA gene expression in the SC-β cells in the absence of contact with the agent and the antioxidant, thereby generating augmented SC-β cells.