METHODS FOR CELL WASHING WITH ON-LINE DILUTION OF CELL FEED

摘要

Systems and methods for the washing of biological fluid/biological cells are disclosed. The method provides for the automated dilution of the cell feed during the cell washing procedure using a separator in which the separator has a predetermined maximum output concentration for the biological cells that are being washed. The method further includes determining the concentration ratio of the biological cells to be washed in the washing procedure and determining a maximum input concentration as a function of the maximum output concentration and the concentration ratio. Wash solution is then added to dilute said biological cells so that the maximum input concentration of the diluted biological cells entering the separator is not exceeded.

主权项

1. A method for washing biological cells with a separator comprising a relatively rotatable cylindrical housing and an internal member, wherein said cylindrical housing has an interior surface and said internal member has an exterior surface, said surfaces defining a gap therebetween, wherein at least one of said surfaces includes a porous membrane and for which a maximum output concentration that can be processed by the separator has been established, the method comprising:
determining a concentration ratio of the biological cells to be washed in the washing procedure; determining a maximum input concentration as a function of the maximum output concentration and the concentration ratio; drawing biological cells from a container in flow communication with said separator; adding wash solution to dilute said biological cells so that the maximum input concentration is not exceeded; introducing said diluted cells into said gap of said separator; rotating at least one or both of said housing and said internal member; separating said cells from at least some of said wash solution; concentrating said cells; removing at least some of said concentrated cells from said separator through a first outlet; and removing at least some of said separated liquid medium from said separator through a second outlet.