| 发明名称 |
METHOD FOR TARGETED MODIFICATION OF ALGAE GENOMES |
| 摘要 |
The invention relates to a method for modifying genetic material in algal cells that includes the use of rare-cutting endonuclease to target specific genomic sequences. In particular, the invention relates to a method for modifying genetic material in algal cells wherein rare-cutting endonuclease, especially a homing endonuclease or a TALE-Nuclease, is expressed over several generations to efficiently modify said target genome sequences. |
| 申请公布号 |
US2016272980(A1) |
申请公布日期 |
2016.09.22 |
| 申请号 |
US201314442323 |
申请日期 |
2013.11.18 |
| 申请人 |
TOTAL MARKETING SERVICES |
发明人 |
DUCHATEAU Philippe;DABOUSSI Fayza |
| 分类号 |
C12N15/82;C12N15/52;C07K14/195;C12N9/22;C12N9/12;C12R1/89 |
主分类号 |
C12N15/82 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for targeted modification of the genetic material of an algal cell comprising the steps of:
a) selecting a nucleic acid target sequence in the genome of an algal cell; b) designing a gene encoding a rare-cutting endonuclease to target this sequence; c) transfecting algal cells with one or more vectors comprising said gene encoding said rare-cutting endonuclease to obtain its expression within said cell over several generations; d) selecting the cell progeny of said algal cells having a modified target sequence. |
| 地址 |
Puteaux FR |
