| 发明名称 | Intein-modified enzymes, their production and industrial applications | ||
| 摘要 | A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided. | ||
| 申请公布号 | US9464333(B2) | 申请公布日期 | 2016.10.11 |
| 申请号 | US201013508234 | 申请日期 | 2010.11.05 |
| 申请人 | Agrivida, Inc. | 发明人 | Apgar James;Lessard Philip;Raab R. Michael;Shen Binzhang;Lazar Gabor;de la Vega Humberto |
| 分类号 | C12N9/48;C12N9/42;C12N9/24;C12N15/82 | 主分类号 | C12N9/48 |
| 代理机构 | Volpe and Koenig, P.C. | 代理人 | Volpe and Koenig, P.C. |
| 主权项 | 1. An intein modified protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOS: 2374, 2376-2378, 2383, 2385-2386, 2410, 2412-2413, 2420, 2422-2425, 2428, 2430-2431, 2436, 2442-2444, 2451, 2454-2458, 2460-2461, 2465-2468, 2471-2474, 2477-2480, 2482-2483, 2493-2494, 2500-2501, 2504, 2507-2513, 2517-2519, 2530-2531, 2533-2537, 2539-2543, 2545, 2548-2549, 2555-2557, 2559, 2565, 2569-2571, 2573-2575, 2579-2582, 2584-2597, 2600, 2602-2605, 2607-2621, 2624-2626, 2629-2634, 2636-2639, 2643, 2645-2648, 2650, 2652, 2656-2658, 2661, 2664, 2666-2672, 2674, 2677-2683, 2685, and 3315-3322. | ||
| 地址 | Medford MA US | ||