| 摘要 |
PROBLEM TO BE SOLVED: To provide a chromatographic separation method for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture which is a fish oil or derivative thereof.SOLUTION: A method includes steps of: (i) purifying a feed mixture in a chromatographic separation step to obtain a first intermediate product; (ii) purifying the first intermediate product, obtained in the step (i), in a simulated or actual moving bed chromatographic separation step to obtain a second intermediate product; and (iii) purifying the second intermediate product, obtained in the step (ii), in a simulated or actual moving bed chromatographic separation step to obtain the PUFA product. Saturated and/or monounsaturated fatty acids present in the feed mixture are removed in the first separation step, and the PUFA product is isolated in the steps (ii) and (iii), the PUFA product containing EPA or a derivative thereof in an amount greater than 90 wt.%. An aqueous organic solvent is used as eluent in each separation step.SELECTED DRAWING: None |
