| 摘要 |
A substrate for surface enhanced Raman scattering (SERS) is described in which the analyte is deliberately sandwiched between a colloidal metal nanoparticle and the SERS substrate. In a first embodiment, the analyte is a protein, and the sandwich comprises a protein:Au colloid complex adsorbed to an aggregated Ag sol. Another embodiment concerns a protein:Ag complex, adsorbed to an aggregated Ag sol. In another embodiment, a protein:Au colloid complex is covalently or non-covalently attached to a macroscopic SERS-active substrate. In yet another embodiment, the colloidal nanoparticle is stabilized by a polymeric additive. In yet another embodiment, detection is accomplished at extremely low analyte concentrations. In a final embodiment, control of analyte orientation with respect to the SERS substrate is demonstrated.
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