发明名称 |
INTEIN-MODIFIED ENZYMES, THEIR PRODUCTION AND INDUSTRIAL APPLICATION |
摘要 |
A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided. |
申请公布号 |
US2016186157(A1) |
申请公布日期 |
2016.06.30 |
申请号 |
US201615002280 |
申请日期 |
2016.01.20 |
申请人 |
AGRIVIDA, INC. |
发明人 |
SHEN BINZHANG;LAZAR GABOR;DE LA VEGA HUMBERTO;APGAR JAMES;LESSARD PHILIP;RAAB R. MICHAEL |
分类号 |
C12N9/24;C12N15/82;C12N9/42 |
主分类号 |
C12N9/24 |
代理机构 |
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代理人 |
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主权项 |
1. An intein modified protein comprising a target protein and an intein inserted into the target protein at a site i) immediately prior to a cysteine, threonine, or serine residue of the target protein, ii) within ten angstroms from an active site of the target protein, and iii) within 10 amino acids of a loop-β-sheet junction or within 10 amino acids of a loop-α-helix junction of the target protein, wherein the target protein is a xylanase or a cellulase. |
地址 |
MEDFORD MA US |