| 发明名称 |
VARIANT ENZYMES |
| 摘要 |
Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described. |
| 申请公布号 |
US2016186155(A1) |
申请公布日期 |
2016.06.30 |
| 申请号 |
US201414909041 |
申请日期 |
2014.07.24 |
| 申请人 |
DANISCO US INC. |
发明人 |
DOUGLAS Nicholai;FOUKARAKI Maria;HOMMES Ronaldus Wilhelmus;KAPER Thijs;KELEMEN Bradley R.;KRALJ Slavko;LANTZ SUZANNE E.;LASSILA Jonathan K.;NIKOLAEV Igor;TORRES-PAZMINO Daniel Esteban;VAN DER KLEY Wilhelmus;VAN LIESHOUT Johannes Franciscus Thomas;VAN STIGT THANS SANDER |
| 分类号 |
C12N9/42;C12P19/02;C12P19/14 |
主分类号 |
C12N9/42 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A variant of a parent glycoside hydrolase family 61 (GH61) enzyme, wherein said variant has cellulase activity, has at least 80% sequence identity to SEQ ID NO:3, and has at least one improved property over said parent GH61 enzyme selected from: (a) expression, (b) thermostability and/or Tm, (c) performance in a Whole Hydrolysate Dilute Acid Pretreated Corn Stover (whPCS) hydrolysis assay, and (d) performance in a Dilute Ammonia Pretreated Corn Stover (daCS) hydrolysis assay, wherein said variant comprises at least one amino acid substitution at one or more position corresponding to amino acids 111 to 114 and 144 to 163 of SEQ ID NO:3. |
| 地址 |
Palo Alto CA US |
