Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences

摘要

An apparatus for chip-based sorting, amplification, detection, and identification of a sample having a planar substrate. The planar substrate is divided into cells. The cells are arranged on the planar substrate in rows and columns. Electrodes are located in the cells. A micro-reactor maker produces micro-reactors containing the sample. The micro-reactor maker is positioned to deliver the micro-reactors to the planar substrate. A microprocessor is connected to the electrodes for manipulating the micro-reactors on the planar substrate. A detector is positioned to interrogate the sample contained in the micro-reactors.

主权项

1. An apparatus for chip-based sorting and detection of a sample, consisting of:
a single planar substrate, said single planar substrate having a single planar surface that is divided into cells; said cells arranged on said single planar surface of said single planar substrate in multiple rows and multiple columns wherein said multiple rows are parallel and wherein said multiple columns are parallel and wherein said multiple rows and said multiple columns intersect; electrodes in said cells in said multiple rows and multiple columns on said single planar substrate; a carrier fluid in a carrier fluid source wherein said carrier fluid is directed onto said single planar surface of said single planar substrate and wherein said carrier fluid washes all of said single planar surface of said single planar substrate, a droplet generator connected to said carrier fluid source and said carrier fluid, a sample source containing the sample connected to said droplet generator; wherein said carrier fluid, said droplet generator, and the sample from said sample source produce micro-reactors containing the sample in said carrier fluid with said micro-reactors delivered onto said single planar surface of said single planar substrate; a microprocessor connected to said electrodes in said cells for manipulating said micro-reactors on said single planar surface of said single planar substrate and directing said micro-reactors containing the sample in said carrier fluid into said cells in said multiple rows and multiple columns on said single planar surface of said single planar substrate using said electrodes, and a detector positioned to interrogate the sample contained in said micro-reactors on said cells in said multiple rows and multiple columns on said single planar surface of said single planar substrate.