发明名称 |
METHOD FOR QUANTITATIVE MONITORING OF ENDOSPORES IN AQUEOUS ENVIRONMENT OF A PAPER OR BOARD MILL |
摘要 |
The invention relates to a method for quantitative monitoring of bacterial endospores in an aqueous environment of a paper or board mill. The method comprises at least the following steps: obtaining at least a first aqueous sample originating from the industrial aqueous environment; destroying bacteria in vegetative form in the first sample by a suitable treatment, preferably by heating the first sample to a desired temperature; adding intercalating agent (such as PMA) to the treated first sample and allowing it to interact (e.g. by cross-linking) with the destroyed bacteria, so that the nucleic acid from the destroyed bacteria are unavailable for PCR; and determining the endospore level in the first sample by using quantitative polymerase chain reaction (qPCR) in which only the DNA from the endospores is available for amplification. |
申请公布号 |
WO2016135387(A1) |
申请公布日期 |
2016.09.01 |
申请号 |
WO2016FI50123 |
申请日期 |
2016.02.26 |
申请人 |
KEMIRA OYJ |
发明人 |
RIIHINEN, Kalle;LAURAEUS, Marko;KOLARI, Marko;AHOLA, Juhana |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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