Vectors for directional cloning

摘要

The invention provides vectors and methods for directional cloning.

主权项

1. A method of inducing expression of a DNA sequence of interest in a host cell, comprising contacting a recombinant host cell which is deficient in rhamnose catabolism, and has a recombinant DNA molecule comprising a rhamnose-inducible promoter operably linked to an open reading frame for a heterologous RNA polymerase, with rhamnose and an expression vector comprising a promoter for the heterologous RNA polymerase operably linked to a DNA sequence of interest, so as to induce expression of the DNA sequence of interest, wherein the host cell is an E. coli cell.