| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a stem cell characterized by the ability to renew and the ability to form endothelial and/or endothelial-like cells, a method for isolating the stem cell and the use of the cell. <P>SOLUTION: The method for culturing a P1H12+ endothelial stem cell comprises (a) contact of a population of human peripheral blood mononuclear cell (PBMC) containing the P1H12+ endothelial stem cell with a molecule specifically binding to P1H12+ under a condition to bind the molecule to the P1H12+ endothelial stem cell, (b) separation of the P1H12+ endothelial cells bound to the molecule from PBMC cells which are not bound to the molecule, (c) incubation of the separated P1H12+ endothelial stem cell in a medium in contact with a surface covered with collagen, and (d) the removal of the separated P1H12+ endothelial stem cells which are not bound to the collagen-covered surface from the medium. <P>COPYRIGHT: (C)2008,JPO&INPIT |
