Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. The enhanced rate of mutation can be further augmented using mutagens. Moreover, the hypermutability of mismatch repair deficient cells can be remedied to stabilize cells or mammals with useful mutations.
申请公布号
WO0188192(A2)
申请公布日期
2001.11.22
申请号
WO2001US15376
申请日期
2001.05.14
申请人
THE JOHNS HOPKINS UNIVERSITY;MORPHOTEK INC.;NICOLAIDES, NICHOLAS, C.;SASS, PHILIP, M.;GRASSO, LUIGI;VOGELSTEIN, BERT;KINZLER, KENNETH, W.
发明人
NICOLAIDES, NICHOLAS, C.;SASS, PHILIP, M.;GRASSO, LUIGI;VOGELSTEIN, BERT;KINZLER, KENNETH, W.