System and device for characterizing cells

摘要

A diagnostic device includes a microscope configured to obtain image data on a plurality of cells and a computing device. The computing device is configured to receive the image data, identify at least a portion of each of the plurality of cells based on the received image data, determine at least one of a value of a morphological parameter for each identified at least a portion of the plurality of cells or a relative organization among the identified at least a portion of the plurality of cells, and calculate statistics for the plurality of cells based on the at least one of the determined values of the morphological parameter or the determined relative organization, the statistics including information suitable for distinguishing metastatic cells from non-metastatic cells. The diagnostic device further includes an output device configured to output the statistics for diagnosis.

主权项

1. A microscopy device comprising:
a microscope configured to obtain image data on a plurality of cells in different cell cycle phases on a substrate; a computing device configured to:
receive the image data;normalize the received image data;identify at least a portion of each of the plurality of cells based on the normalized image data;determine a value of a morphological parameter for each identified at least a portion of each of the plurality of cells; anddetermine values of the morphological parameter for corresponding cell cycle phases and fractions of the plurality of cells in the corresponding cell cycle phases based on the determined value of the morphological parameter for each identified at least a portion of each of the plurality of cells; and an output device configured to output the values of the morphological parameter for corresponding cell cycle phases and fractions of the plurality of cells in the corresponding cell cycle phases, wherein identifying at least a portion of each of the plurality of cells based on the normalized image data comprises segmenting at least a portion of each of the plurality of cells based on the normalized image data comprises segmenting nuclei of the plurality of cells in the normalized image data, wherein segmenting the at least a portion of each of the plurality of cells comprises: obtaining Gaussian intensity image data of the normalized image data; obtaining averaged intensity image data of the normalized image data; obtaining nuclear intensity image data based on the difference between the Gaussian intensity image data and the averaged intensity image data; and segmenting nuclei based on the nuclear intensity image data.