Chip set-up and high-accuracy nucleic acid sequencing

摘要

The present disclosure provides devices, systems and methods for sequencing nucleic acid molecules. Nucleic acid molecules can be sequenced with a high accuracy (e.g., greater than 97% in a single pass) using a chip comprising an array of independently addressable nanopore sensors at a density of at least about 500 sites per 1 mm2. An individual nanopore sensor can include a nanopore in a membrane that is adjacent or in proximity to a sensing electrode.

主权项

1. A device for use in molecular sensing comprising one or more nanopores in a membrane disposed upon at least one membrane incompatible surface in sensing proximity to an electrode coupled to an integrated circuit, wherein said membrane comprising said one or more nanopores exhibits a capacitance greater than about 5 fF/μm2 and a conductance less than about 10 nano Siemens per mole of electrolyte per nanopore as measured by said electrode under an applied voltage of at least about 50 mV.