| 摘要 |
PURPOSE:To quantity a thiol group with high accuracy, by reacting 2,4- dinitrophenylselenocyan with a thiol compound to quantitatively form 2,4- dinitroselenophenol and performing the S-cyanization of the thiol group within a short time. CONSTITUTION:2,4-dinitrophenylselenocyan is reacted with a thiol compound being protein or peptide to specifically form an S-cyano derivative and 2,4- dinitroselenophenol simultaneously released is quantified. This S-cyanization reaction of the thiol group is completed within 3min, and reaction and quantification are simultaneously and rapidly performed to quantify the thiol group with good accuracy and peptide segmentation treatment can be continuously performed.
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