MULTIMERIC PROTECTED FLUORESCENT REAGENTS

摘要

Multimeric protected fluorescent reagents and their methods of synthesis are provided. The reagents are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The reagents contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements. The reagents also contain coupling elements connect monomeric compounds into multimeric forms, thereby increasing brightness.

主权项

1. A reagent of structural formula (IV):
J-[-B″—S″—Z—[—S′—B′]m′]q   (IV); wherein each Z is independently a multivalent central core element comprising a fluorescent dye element; each S′ is independently an intermediate chemical group, wherein at least one S′ comprises a shield element; each S″ is independently an intermediate chemical group, wherein S″ optionally comprises a shield element; each B′ is independently a terminal chemical group, wherein at least one B′ comprises a binding element; each B″ is independently a terminal chemical group or a bond; J is a joining element; each m′ is independently an integer from 1 to 23; and q is an integer from 2 to 10.