| 摘要 |
PCT No. PCT/US92/08835 Sec. 371 Date May 10, 1993 Sec. 102(e) Date May 10, 1993 PCT Filed Oct. 15, 1992.A method is provided for the preparation and/or analysis of synthetic phosphorothioate and -dithioate oligonucleotides. In particular, the method permits separation of fully sulfurized phosphorothioate or -dithioate oligonucleotides from incompletely sulfurized defect species on an anion exchange HPLC columns using concentration gradients of novel "soft base" anionic eluents, such as bromide, thiocyanate, and the like.
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