摘要 |
<p>Screening methods for identifying compounds that inhibit the ubiquitination reaction mediated by the APC are based on the ability of the APC subunit APC11 to form multiubiquitin chains. A compound's ability to interfere with self-ubiquitination of APC11, with the formation of multiubiquitin chains independent of their attachment to APC11 or with ubiquitination of an APC substrate can be measured.</p> |