发明名称 |
PRODUCTION HOST CELL LINES |
摘要 |
The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a DHFR expression cassette. Those cell lines have improved growth characteristics in comparison to DHFR-deficient or DHFR-reduced cell lines such as CHO DG44 and CHO DUKX-B11. The invention especially concerns two cell lines, a representative of each cell line is deposited with the DSMZ under the number DSM ACC2909 (CHOpper(R) Discovery) and DSM ACC2910 (CHOpper(R) Standard). The invention further concerns a method of producing proteins using the cells generated by the described method.
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申请公布号 |
US2010021911(A1) |
申请公布日期 |
2010.01.28 |
申请号 |
US20090507252 |
申请日期 |
2009.07.22 |
申请人 |
BOEHRINGER INGELHEIM PHARMA GMBH & CO KG |
发明人 |
KAUFMANN HITTO;FLORIN LORE;BECKER ERIC;STUDTS JOEY M. |
分类号 |
C12Q1/68;C12N5/10;C12N5/16;C12N15/85;C12P21/02 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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