| 摘要 |
PURPOSE:To efficiently produce bacterium cellulose by using a spinner culture tank having a specific coefficient of shear rate, culturing a bacterium capable of producing cellulose under a stirring condition of a specific oxygen transfer rate to increase production rate and maximum accumulative concentration of bacterium cellulose. CONSTITUTION:A stirring shaft rotatable out of a spinner culture bath is installed at the central part of the spinner culture tank, the shaft is equipped with a bottom paddle, having a lower part in close vicinity to the bottom of the spinner culture tank, arranged at the bottom the tank and an arm paddle and a strip extending in the direction of a shaft are attached to a part higher than the bottom of paddle of the stirring shaft to constitute a lattice blade. Plural baffle plates set along the axial direction from the bottom to the top are attached at intervals to a side wall face of the spinner culture tank to give the spinner culture tank having >=15 coefficient of shear rate. A bacterium capable of producing cellulose is cultured by using the spinner culture tank under a stirring condition of >=0.5X10<6>mol/ml/minute to efficiently produce bacterium cellulose at increased production rate and in the increased maximum accumulative concentration. |
